Agronomic Evaluations of New Transgenic and Non Transgenic Cotton Varieties in
La Paz and Mohave Counties
Tim C. Knowles, La Paz County Cooperative Extension
Del Wakimoto, Farmer, Mohave Valley, AZ
Greg Sprawls, Farmer, La Paz County, AZ
Greg Hurtado, La Paz County Cooperative Extension
In 1996 transgenic Bt cotton was first grown on a commercial level in Arizona.
In 1997 transgenic Roundup and Buctril herbicide resistant cotton varieties were
introduced and grown on commercially in Arizona. Furthermore, in 1997, four new
heat tolerant non-transgenic cotton varieties for commercial release in 1998 were
available for University field tests. Prior to 1997, the agronomic
characteristics of these new cotton varieties had not been evaluated in side by
side replicated field comparisons beyond the level of the developing companies.
Field tests were conducted in La Paz and Mohave Counties in 1997 examining
agronomic characteristics of new transgenic Bt (Deltapine 5415 vs. Deltapine 32 B
and 33B) and herbicide resistant (Paymaster 1220 BGRR vs. 1244 BGRR and
Stoneville 474 vs. BXN 47) and non transgenic heat tolerant (SureGrow 125 vs.
180 and 821, Stoneville 474 vs. 468, and Germain's GC 9230) cotton varieties.
Spring 1996 was the first year Deltapine (DP) transgenic Bt cotton was
commercially available in Arizona. Companion varieties DP 5415 and NuCotn 33B
were evaluated for agronomic performance (ie. growth, development, lint quality
and yield) at nine locations across cotton producing regions of Arizona
(Silvertooth et. al., 1997). Only slightly higher vigor or growth rates were
noted for 33B over 5415, and higher lint yields for 33B over 5415 were observed
in most cases. It was concluded that 33B, as a transgenic version of 5415, is
indeed very close in agronomic performance to its non-Bt counterpart.
In 1997, Deltapine commercially introduced another new transgenic cotton
variety called NuCotn 32B, which is a companion variety to DP 5432 and 5415.
Spring 1997 was the first year transgenic cotton varieties with the stacked Bt
(Bollgard or BG) plus Roundup Ready (RR herbicide resistant) genes were
commercially available in two new varieties, Paymaster 1220 BGRR and 1244
BGRR which were companion varieties to 1220 and 1244, respectively . Prior to
this introduction, Paymaster cotton varieties had been grown only on a limited
number of Arizona farms. Additionally, in 1997, Stoneville released a new
transgenic Buctril herbicide resistant variety called BXN 47 which was a
companion variety to 474. Insecticidal or herbicidal properties of these new
transgenic varieties and their non-transgenic counterparts were evaluated
extensively prior to their release, however their agronomic properties had not
been evaluated to any sufficient extent beyond the level of the developing
Heat stress has been assigned much of the blame for the relatively poor
performance of low desert cotton crops in recent years. In 1997, four new
cotton advanced strains with potential for heat tolerance were available for
University of Arizona (UA) evaluations of agronomic performance. These new
varieties should be commercially available in 1998 and included SureGrow 180
and 821 which were compared to 125, Stoneville 468 which was compared to 474,
and Germain's GC 9230.
The objectives of this study were to conduct side by side replicated agronomic
evaluations of: 1) two transgenic Bt cotton varieties (DP 32B and 33B), compared
to their non-Bt counterpart (DP 5415); 2) two transgenic stacked Bt and Roundup
Ready (RR) cotton varieties (Paymaster 1220 BGRR and 1244 BGRR), compared to DP
32B and 33B; 3) one transgenic Buctril resistant cotton variety (Stoneville BXN
47), compared to its non-BXN counterpart (Stoneville 474) and the varieties
examined in objectives 1 and 2 above; and 4) four new heat tolerant non-transgenic
cotton varieties (SureGrow 180 and 821, Stoneville 821, and Germain's 9230),
compared to their recurrent or non-transgenic parents (SureGrow 125 and
Stoneville 474) and the varieties outlined in objectives 1 through 3 above.
Materials and Methods
Two experiments associated with the UA upland cotton variety testing program
was conducted during 1997 in Parker Valley (located in southwestern La Paz
County) and Mohave Valley (located in western Mohave County).
Greg Sprawls, farm manager for CRIT Farms was the Parker Valley site
cooperator. Deltapine 5415, 32B, and 33B; Paymaster 1220 BGRR and 1244 BGRR;
Stoneville 474 and BXN 47; SureGrow 125 and 180; and Germain's GC 9230 were
planted wet on April 11 with a John Deere 7300 Maxi-Merge Vacuum Plate Planter
that hill dropped 3 seed every 10 inches of row. Each variety was replicated four
times in a randomized complete block experimental design. Individual plots were 4
rows wide (40 inch rows) by the length of the irrigation run (1250 feet long).
The field was managed by the grower-cooperator. A complete set of plant
measurements were collected on monthly intervals. Measurements taken included:
initial population, plant height, number of mainstem nodes, first fruiting branch,
total number of aborted sites (1st and 2nd position), and the number of nodes
above the uppermost white bloom. Seed cotton yields were estimated by
mechanically harvesting each plot, and determining plot weights by the
use of electronic scales placed at the end of the field on October 23.
Subsamples of seed cotton were ginned for lint turnout estimates and lint
samples were subjected to HVI analysis. Data was analyzed statistically in
a manner consistent with the experimental design using ANOVA methods.
Del Wakimoto, farm manager for Ft. Mojave Tribe Avi Kwa ĎAme Farms was
the Mohave Valley site cooperator. Deltapine 32B and 33B;
Paymaster 1220 BGRR and 1244 BGRR; Stoneville 474 and 468; and SureGrow 125
and 821 were planted wet on April 9 with a John Deere 7100 Maxi-Merge Planter
calibrated for 14 lbs Deltapine 33B seed per acre. Each variety was replicated
four times in a randomized complete block experimental design. Individual plots
were 6 rows wide (40 inch rows) by the length of the irrigation run (810 feet
long). A complete set of plant measurements were collected on monthly intervals.
Measurements taken included: initial population, plant height, number of mainstem
nodes, first fruiting branch, total number of aborted sites (1st and 2nd
position), and the number of nodes above the uppermost white bloom. Seed cotton
yields from four center rows of each plot were determined on October 16.
Results and Discussion
Cotton plant populations at the Parker Valley site were minimal ranging from
19,675 to 24,725 plants/acre. From the plant mapping data presented in
4 for Parker Valley
and Tables 5,
and 8 for Mohave Valley,
evidence is presented that supports the claims that DP 32B and 33B are indeed very similar to
their non-Bt counterpart, DP 5415. Although plant vigor was relatively high at both sites, no
differences were detected with respect to vigor or growth rates and fruit retention when
comparing DP 5415, 32B, and 33B. However, at the Parker Valley location, DP 33B plant
populations were approximately 10% lower than DP 5415 and 32B seeded at identical rates.
This supports some field observations during the 1997 cotton growing season in these valleys
indicating that DP 33B planted into cool soil had slightly lower seedling vigor compared to DP
Paymaster 1220 BGRR and 1244 BGRR was 2 to 5 inches taller and had 15 to 25% higher height
to node ratios through the peak bloom growth stage compared to DP 5415, 32B, and 33B. In fact,
Paymaster 1220 BGRR and 1244 BGRR had the highest vigor or growth rates prior to peak bloom
compared to all the other cotton varieties examined in this study. Paymaster 1244 BGRR was
slightly more vigorous in growth rate compared to 1220 BGRR. These two relatively large
seeded varieties also had lower final plant populations compared to DP 5415, 32B, and 33B.
Both the Paymaster 1220 BGRR and 1244 BGRR could have possibly benefitted from early to mid
season growth regulator (Pix) applications due to low fruit retention and high height to node
A fair evaluation of heat tolerance can be made by comparing August and September fruit
retention levels among the upland cotton varieties tested in this study. There were no
significant differences observed among the varieties examined in this study in fruit
retention levels at the 1st and 2nd positions during August and September. Fruit retention
levels for all varieties ranged from 42.6 to 57.4% during this time. This would indicate
that the varieties examined in this study were similar in their ability to tolerate the
relatively minimal heat stress encountered during the 1997 growing season.
At the Parker Valley site, lint yields of the two DP transgenic Bt cotton varieties
(32B and 33B) were not significantly different from each other or from their parent non-Bt
variety (5415) at the October 23 harvest (Table 4).
Of the two transgenic stacked Bt and RR cotton varieties, lint yield of Paymaster 1244 BGRR
was comparable to the DP Bt and non-Bt cotton varieties, however lint yield of Paymaster 1220
BGRR was lower than DP 32B. Lint yield of Stoneville 474 was statistically equivalent to its
transgenic Buctril resistant counterpart BXN 47. Nevertheless, lint yield of Stoneville 474
was significantly higher compared to the other cotton varieties grown in this test (DP 5415,
32B, and 33B; Paymaster 1220 BGRR and 1244 BGRR; and SureGrow 125). SureGrow 125 had the
lowest lint yields observed in this study, possibly due to a late replant of one row in
each plot caused by a clogged planter hose.
At the Mohave Valley site, lint yields of the two DP transgenic Bt cotton varieties
(32B and 33B) were not significantly different from each other at the October 16 harvest
(Table 8). Of the two transgenic stacked Bt and RR cotton
varieties, lint yield of Paymaster 1244 BGRR was comparable to the DP Bt varieties
(33B and 35B), however lint yield of Paymaster 1220 BGRR was lower than that of the other
varieties examined in this study (DP 32B and 33B; SureGrow 125 and 821; and Stoneville 474
and 468). The heat tolerant SureGrow 832 did not significantly outyield its counterpart,
SureGrow 125. Another heat tolerant cotton variety, Stoneville 468, had lint yield that was
not significantly different from its counterpart, Stoneville 474.
At both sites, DP 5415, 32B, and 33B lint yields were not significantly different,
indicating that they are quite similar in yield, growth habit, and fruit retention. Paymaster
1220 BGRR had lower lint yields than Paymaster 1244 BGRR, however lint yield of Paymaster 1244
BGRR was comparable to that of the DP varieties examined in this study (5415, 32B, and 33B).
Lint yield of Stoneville 474 was comparable to or greater than that of the DP varieties, and
lint yields of Stoneville BXN 47 and 468 were comparable to Stoneville 474. Lint yield of
SureGrow 821 was comparable to SureGrow 125, however SureGrow 821 seemed to be somewhat
more heat tolerant than SureGrow 125. Yield comparisons of the varieties examined in this
study were conducted under a relatively short growing season and light pink bollworm pressure.
Consequently, their relative lint yields could be somewhat different under higher pink
bollworm infestations or when subjected to full or long season production systems.
Silvertooth, J.C., E.R. Norton, S.H. Husman, T. Knowles, and D. Howell. 1997.
Agronomic evaluations of Bt cotton. Cotton, A College of Agriculture Report.
University of AZ Series P-108. pp. 31-40.
The valuable cooperation, land, and resources provided by Del
Wakimoto of Avi Kwa ĎAme Farms and Greg Sprawls of CRIT Farms is highly
appreciated. The support and cooperation provided by the participating
companies (Deltapine, Paymaster, SureGrow, Stoneville, and Germain's) is
This is a part of publication AZ1006:
"Cotton: A College of Agriculture Report," 1998, College of Agriculture, The University of
Arizona, Tucson, Arizona, 85721.
Any products, services, or organizations that are mentioned, shown, or indirectly
implied in this publication do not imply endorsement by The University of Arizona.
The University is an Equal Opportunity/Affirmative Action Employer.
This document located at http://ag.arizona.edu/pubs/crops/az10063k.html
Return to Cotton 98 index