Whitefly Management in Arizona: Conservation of Natural Enemies Relative to Insecticide Regime

Steven E. Naranjo, ARS Western Cotton Research Laboratory, Phoenix
James R. Hagler, ARS Western Cotton Research Laboratory, Phoenix
Peter C. Ellsworth, Maricopa Agricultural Center

Abstract

Field studies were conducted in 1997 to evaluate strategies for management of whitefly (Bemisia tabaci). We evaluated the effects of different insecticide regimes (conventional and insect growth regulators [IGR]) on the abundance of native parasitoids and predators associated with whitefly in Arizona cotton. Immature parasitoids were most abundant in untreated control plots and there was little difference among insecticide regimes. Percentage parasitism was low overall (< 30%), but was highest in Knack plots and lowest in untreated control and Applaud plots. Predator populations were lowest in plots treated with conventional insecticides, and there were several instances where weekly or season-long populations of several predator species/groups were slightly depressed in IGR plots compared with the untreated check. Overall, results are encouraging and indicate that use of IGRs helps to conserve populations of native natural enemies.

Introduction

Significant advances have been made in managing whiteflies in Arizona cotton over the past few years. Sampling methods and action thresholds have been developed for efficiently timing the use of conventional insecticides and insect growth regulators (IGRs) (see Ellsworth et al. 1998). In part, these advances also have prompted renewed interest in examining and improving our understanding of the role of natural enemies in cotton pest management. Many species of beneficial arthropods naturally inhabit cotton fields and it is generally thought that they play an important role in maintaining many potential cotton pests (e.g., cotton leafperforator, beet armyworm) at harmless levels. They also may contribute to control of key cotton pests like whitefly, Lygus and pink bollworm. In 1996 we initiated studies to examine natural enemy conservation in relation to different whitefly management practices. These commercial-scale trials suggested that the use of the IGRs Applaud (buprofezin) and Knack (pyriproxyfen) may conserve populations of predators and whitefly parasitoids (Naranjo and Hagler 1997). These studies were continued in 1997 under quasi-commercial conditions at the Maricopa Agricultural Center. We report here the comparative population dynamics of native parasitoids and predators relative to different whitefly management systems. The overall whitefly management trial is described by Ellsworth (1998) and detailed studies to estimate causes and rates of whitefly mortality due to natural enemies and other factors are presented in Naranjo et al. (1998).

Materials and Methods

The study was conducted using NuCOTN 33B and contrasted four whitefly control regimes; Applaud used first, Knack used first, a rotation of conventional insecticides (1995-IRM), and an untreated control. The threshold for first and second IGR treatment was 1 large nymph/disk plus 3-5 adults/leaf (Ellsworth et al. 1996). A rotation of conventional insecticides were used as needed after both IGRs were applied. All conventional insecticide applications were made at 5 adults/leaf (Ellsworth et al. 1995). One insecticide application was made for Lygus hesperus over the entire experiment with Vydate C-LV (1 lb ai/A) on 25 July. All applications were made by ground and seasonal usage of insecticides is summarized in Table 1. Each insecticide regime was replicated 4 times using a randomized block design in a total area of about 8 acres. Additional detail on the entire experiment is provided in Ellsworth et al. (1998).

Parasitoid abundance and activity was estimated by taking weekly leaf samples (20-30 per plot) from the 7th mainstem node from the terminal. In the laboratory we counted all larval and pupal parasitoids of each genus (Eretmocerus and Encarsia) and all unparasitised 4th instar whitefly nymphs on the entire leaf. We calculated an index of parasitism based on the percent 4th instar nymphs parasitized. The abundance of arthropod predators was estimated weekly in each plot with standard 38 cm sweepnets. Twenty-five sweeps were taken in each of two locations for a total of 50 sweeps in each plot. We recorded the abundance of approximately 30 species of arthropod predators from our samples. For week by week analyses we combined predators into 4 broad groups: beetles, spiders, true bugs (excluding Lygus) and other (primarily of green lacewing larvae and a small predatory fly). We further analyzed the mean weekly abundance over the season for all major predator species/groups

Results and Discussion

All plots were treated Lygus on 25 July (Table 1). The first insecticide applications for whitefly control were made 29 July. Whitefly densities increased late season, and conventional materials were necessary in IGR by early September. A total of 4 applications were used in IGR treatments and a total of 5 applications were needed in the conventional treatment for whitefly control.

Eretmocerus eremicus and Encarsia meritoria, two native whitefly parasitoids were present throughout the season. Eret. eremicus was dominant, comprising about 80% of all parasitoids collected. Rates of parasitism were low in the early season, peaked (20-30%) in mid August and declined into September. Parasitism averaged about 8.4% over the whole experimental area for the entire season. Immature parasitoid abundance differed significantly among insecticide regimes on many post-treatment dates and were consistently highest for the untreated control (Figure 1). There was relatively little separation among insecticide treatments which largely reflects the high levels of whitefly control resulting in few hosts available for parasitoid development. Percentage parasitism of 4th instar whitefly differed among treatments on 4 of 6 post-treatment dates and was generally highest for Knack plots and lowest for Applaud and untreated control plots (Figure 1). Despite the moderate levels of parasitism observed here, detailed life table studies (Naranjo et al. 1998) indicate that parasitism is a very minor component of overall mortality within a whitefly generation.

Many of the 30 or more species of predators that we collected and recorded occurred at extremely low densities (< 1 per 50 sweeps). To facilitate analysis of predator abundance we pooled species into four broad groups; beetles, spiders, true bugs, and other. Average densities of predaceous beetles were low (< 3 per 50 sweeps) over the whole season and the only significant differences occurred near the end of the season where densities in untreated control plots were higher than in all other treatments on two dates (Figure 2). The remaining predator groups were affected on the majority of post-treatment sampling dates. The abundance of true bugs (e.g. Geocoris, Orius, Nabis etc.) was consistently lower in conventional insecticide plots, and generally higher in the untreated control. Densities of true bugs in IGR plots were fell between those in conventional and untreated plots , especially toward the end of the season. The same pattern was evident for spider abundance, although there was less separation among untreated and IGR plots and overall densities were low (< 6 per 50 sweeps). The other group, which consisted primarily of green lacewing larvae and Drapetis mediata (predaceous fly of adult whiteflies) was the most abundant group, and there were several sampling dates where densities in IGR plots were significantly lower than the untreated control. Densities were consistently lowest in the conventional plots (Figure 2).

Analyses of seasonal means were conducted for 12 species/groups that occurred at moderate to high densities (Table 2). Mean densities of 5 of the 12 species were statistically higher in IGR plots compared with 95-IRM plots and mean densities were significantly greater in untreated control plots compared with 95-IRM plots for 7 species. Mean densities of 2 species (Geocoris punctipes and Drapetis mediata) were significantly higher in control plots compared with IGR plots. There were no differences in mean densities of any species between the two IGR regimes.

The IGRs Applaud and Knack are highly effective in suppressing whitefly populations (Ishaaya and Horowitz 1992, Ellsworth et al. 1997, Ellsworth et al. 1998 ) and their use is generally considered to be compatible with natural enemy conservation (Gerling and Sinai 1994, Jones et al. 1995, Liu and Stansly 1997, Nagai 1990 ). Our evaluations of natural enemy abundance generally support these conclusions. Compared with conventional insecticides, use of IGRs conserved parasitoids and predators. However, in comparison to untreated plots, populations of natural enemies were depressed in IGR plots on several sampling dates and season-long populations of several abundant predators were similarly affected. These effects were small relative to the impact of applying conventional materials and it is unclear whether these reductions were associated with direct toxic effects or associated reductions in prey populations (see Ellsworth et al. 1998). Overall, our results are encouraging and indicate that use of IGRs for suppression of whiteflies helps conserve populations of native natural enemies. Many of the predators we examined are known to prey on whitefly in cotton (Hagler and Naranjo 1994a,b) and observations of whitefly mortality in the field (Naranjo et al., 1998) suggest that conservation may translate into enhanced levels of predation that substantially contribute to overall control of whiteflies in cotton. These studies are discussed in the following paper.

Acknowledgments

We thank Daniel Ashton, Virginia Barkeley, Jeff Cantrell, Marilyn Carey, Sunny Carrington, Rochelle Christensen, Johnny Fearn, Celso Jara, Dan Langhorst, Scott Machtley, Jeanette Martin, Donna Meade, Greg Owens, and Kim Zaremba for expert technical assitance.

References

  1. Ellsworth, P. C, S. E. Naranjo, S. J. Castle, J. R. Hagler & T. J. Henneberry. 1998. Whitefly management in Arizona: Looking at the whole system. This volume.
  2. Ellsworth, P.C., J.W. Diehl, T.J. Dennehy & S.E. Naranjo. 1995. Sampling Sweetpotato Whiteflies in Cotton. IPM Series No. 2. The University of Arizona, Cooperative Extension. Publication #194023. Tucson, AZ. 2 pp.
  3. Ellsworth, P.C., J.W. Diehl & S.E. Naranjo. 1996. Sampling Sweetpotato Whitefly Nymphs in Cotton. IPM Series No. 6. The University of Arizona, Cooperative Extension. Publication #196006. Tucson, AZ. 2 pp.
  4. Ellsworth, P.C., J.W. Diehl, I.W. Kirk & T.J. Henneberry. 1997. Bemisia growth regulators: large-scale evaluation. In P. Dugger & D. Richter[ed.], Proceedings. Beltwide Cotton Conferences, Cotton Insect Research and Control Conference, Nashville, TN. pp. 922-929.
  5. Hagler, J. R. & S. E. Naranjo. 1994a. A qualitative survey of two coleopteran predators of Bemisia tabaci (Homoptera: Alyerodidae) and Pectinophora gossypiella (Lepidoptera: Gelechiidae) using a multiple prey gut content ELISA. Environ. Entomol. 23: 193-197.
  6. Hagler, J. R. & S. E. Naranjo. 1994b. Determining the frequency of heteropteran predation on sweetpotato whitefly and pink bollworm using multiple ELISAs. Entomol. Exp. Appl. 72: 63-70.
  7. Ishaaya, I. & A. R. Horowitz. 1992. Novel phenoxy juvenile hormone analog (pyriproxyfen) suppresses embryogenesis and adult emergence of sweetpotato whitefly. J. Econ. Entomol. 85: 2113-2117.
  8. Jones, W. A., D. A. Wolfenbarger & A. A. Kirk. 1995. Response of adult parasitoids of Bemisia tabaci to leaf residues of selected cotton insecticides. Entomophaga 40: 153-162.
  9. Liu, T. X. & P. A. Stansly. 1997. Effects of pyriproxyfen on three species of Encarsia, endoparasitoids of Bemisia argentifolii. J. Econ. Entomol. 90: 404-411.
  10. Nagai, K. 1990. Effects of a juvenile hormone mimic material, 4-phenoxyphenyl (RS)-2(2-pyridyloxy) propyl ether, on Thrips palmi and its predator Orius spp. Appl. Entomol. Zool. 25: 199-204.
  11. Naranjo, S. E. & J. R. Hagler. 1997. Conservation of natural enemies relative to use of insect growth regulators for control of sweetpotato whitefly. pp. 318-323. In Cotton: A College of Agriculture Report, Series P-108, Univ. Arizona, Tucson.
  12. Naranjo, S. E., P. C. Ellsworth & J. W. Diehl. 1998. Whitefly management in Arizona: Contribution of natural enemies to whitefly mortality. This volume.

This is a part of publication AZ1006: "Cotton: A College of Agriculture Report," 1998, College of Agriculture, The University of Arizona, Tucson, Arizona, 85721. Any products, services, or organizations that are mentioned, shown, or indirectly implied in this publication do not imply endorsement by The University of Arizona. The University is an Equal Opportunity/Affirmative Action Employer.
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