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A defoliated cotton field
Aflatoxin Reduction in Crops (ARC)
Aflatoxin Management through Improved Understanding of Aflatoxin-producing Fungi and the Aflatoxin Contamination Process
Media Recipes
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5-2 (Low Nutrient Utility Medium): Use for Culture Maintenance and for growing fungi prior to storage.

AFPA: Use to identify Aspergillic Acid Production and as a taxonomic tool for differentiating species and morphotypes.

A&M Liquid Medium (Adye& Mateles 1964) with supplements: Chemically defined medium for asse4ssing aflatoxin production by Aspergillus isolates

CU (Modified Rose Bengal): For isolation of Aspergillus section flavi from soil and crop

CZ (Czapeks-Dox Agar): General utility medium for fungi

HYP (Hypozanthine Media): For phenotyping nitrate non-utilizing mutants; Cnx mutants cannot utilize hypoxanthene

Micronutrients (A&M): General Micronutrient Solution

MIT (Secondary Selection Media):  Use to “clean up” or purify nit- mutants.

Nit (Nitrite Media): For phenotyping nitrate non-utilizing mutants; NirA and NiaA mutants cannot utilize nitrite

SEL (Selection Media): Use to Select Nitrate Nonutilizing (nit-) mutant

Starch: Complementation media for assessing membershim in a VCG

YES Liquid Medium (2% Yeast extract 15% sucrose): Complex medium for assessing aflatoxin production by Aspergillus isolates

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United States Department of Agriculture
Agricultural Research Service
ARC is housed at the University of Arizona,
Department of Plant Sciences
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The University of Arizona, Department of Plant Sciences, Division of Plant Pathology and Microbiology, PO Box 210036, Tucson, AZ 85721
Current Updates by Gabriel Barker sargasso@email.arizona.edu Tel: (520) 626-5704 Fax: (520) 626-5944