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30g of sterile wheat is inoculated with a spore suspension of isolate AF36 and incubated in 250ml polycarbonate validation bottles at 31C for 7-9 days. During this period the fungus sporulates abundantly. The bottles are fitted with filters that allow gas exchange without loss of moisture. |
| The spores are then washed off into a large water container that will be used to inoculate the product. |
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Clean wheat is stored in an outdoor silo until it is needed. then it is sent up the elevator to a chute above the processor. |
| 6000lbs of wheat is loaded into a double jacketed rotating steam processor where it is sterilized under pressure at 250C for several hours and then cooled. |
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The spore suspension is spread over the sterile wheat in the processor and the processor is rotated for an hour to evenly distribute spores. |
