THE EXTERNAL
SCAFFOLDING PROTEIN
The Four D subunits per asymmetric unit. Each subunit sits
in a different environment and has a different structure. The D4
subunit (blue) is the most structurally unique.
Cross section of the two-fold axis of symmetry. The viral
coat protein is depicted in gray. The external scaffolding proteins are
depicted in green (D1), yellow (D2), red (D3) and blue (D4)
- Current Investigator: Dr. James Cherwa.
- Alumni
Investigators: Pablo Sancez-Soria, Peter Heiman, The 2007 Virology
Laboratory Course at the University of Arizona, Dr. Asako Uchiyama. Min
Chen, Dr.
April D.
Burch-Alden.
- Collaborator: Dr. Michael Rossmann's group (Purdue University).
Dr. Holly Wichman's group (University of Idaho).
Clicking on the link belows
will
retrieve a pdf of the manuscript.
Cherwa, J. E. J., and B. A. Fane. 2009.
Complete virion assembly with
scaffolding proteins altered in the ability to perform a critical
conformational switch. J. Virol. 83: 7391-6. Editors’ Featured
Article.
Cherwa, J. E. J., P. Sanchez-Soria,
The 2007 Undergraduate Virology Laboratory Course, H. Wichman, and B.
A. Fane. 2009. Viral adaptation to an antiviral protein enhances
fitness above the uninhibited wild-type level J. Virol. 83:11746-50.
Uchiyama, A., P. Heiman, and B. A.
Fane. 2009. N-terminal deletions of the phiX174 external scaffolding
protein affect the timing and fidelity of assembly. Virology 386:303-9.
Cherwa, J. E. Jr., Uchiyama, A., Fane B.
A. (2008) Scaffolding proteins altered in the ability to perform a
conformational switch confer dominant lethal assembly defects. J.
Virol. 82: 5774-80. Editors’ Featured Article.
Uchiyama, A., Chen, M., Fane, B. A.
(2007) Characterization and function of putative substrate specificity
domain in Microvirus external scaffolding proteins. J. Virol. 81:
8587-92.
Uchiyama, A., Fane B. A. (2005)
Identification of an interacting coat-external scaffolding protein
domain required for both the initiation of øX174 procapsid
morphogenesis and the completion of DNA packaging. J. Virol. 79:
6751-6.
Morais, M., Fisher, M., Kanamaru, S.,
Fane, B. A., Rossmann, M. G. (2004) Conformational Switching of the
øX174 scaffolding protein D during assembly. Mol. Cell. 15:
991-7.
Burch, A. D., Fane, B. A. (2003) Genetic
analyses of putative conformation switching and cross-species
inhibitory domains in Microviridae external scaffolding proteins.
Virology 310: 64-71.
Burch, A. D., Fane, B. A. (2000)
Foreign and chimeric external scaffolding proteins as inhibitors of
Microviridae morphogenesis. J. Virol. 74: 9347-9352.