The University of Arizona


THE EXTERNAL SCAFFOLDING PROTEIN


 

 

 

 

 

The Four D subunits per asymmetric unit. Each subunit sits in a different environment and has a different structure. The D4 subunit (blue) is the most structurally unique.

 


 

Cross section of the two-fold axis of symmetry. The viral coat protein is depicted in gray. The external scaffolding proteins are depicted in green (D1), yellow (D2), red (D3) and blue (D4)

 




Current Investigator: Dr. James Cherwa.
Alumni Investigators: Pablo Sancez-Soria, Peter Heiman, The 2007 Virology Laboratory Course at the University of Arizona, Dr. Asako Uchiyama. Min Chen, Dr. April D. Burch-Alden.

Collaborator: Dr. Michael Rossmann's group (Purdue University). Dr. Holly Wichman's group (University of Idaho).
Clicking on the link belows will retrieve a pdf of the manuscript.

Cherwa, J. E. J., and B. A. Fane. 2009. Complete virion assembly with scaffolding proteins altered in the ability to perform a critical conformational switch. J. Virol. 83: 7391-6.  Editors’ Featured Article.

Cherwa, J. E. J., P. Sanchez-Soria, The 2007 Undergraduate Virology Laboratory Course, H. Wichman, and B. A. Fane. 2009. Viral adaptation to an antiviral protein enhances fitness above the uninhibited wild-type level J. Virol. 83:11746-50.

Uchiyama, A., P. Heiman, and B. A. Fane. 2009. N-terminal deletions of the phiX174 external scaffolding protein affect the timing and fidelity of assembly. Virology 386:303-9.

Cherwa, J. E. Jr., Uchiyama, A., Fane B. A. (2008) Scaffolding proteins altered in the ability to perform a conformational switch confer dominant lethal assembly defects. J. Virol. 82: 5774-80. Editors’ Featured Article.

Uchiyama, A., Chen, M., Fane, B. A. (2007) Characterization and function of putative substrate specificity domain in Microvirus external scaffolding proteins.  J. Virol. 81: 8587-92.

Uchiyama, A., Fane B. A. (2005) Identification of an interacting coat-external scaffolding protein domain required for both the initiation of øX174 procapsid morphogenesis and the completion of DNA packaging. J. Virol. 79: 6751-6.

Morais, M., Fisher, M., Kanamaru, S., Fane, B. A., Rossmann, M. G. (2004) Conformational Switching of the øX174 scaffolding protein D during assembly. Mol. Cell. 15: 991-7. 

Burch, A. D., Fane, B. A. (2003) Genetic analyses of putative conformation switching and cross-species inhibitory domains in Microviridae external scaffolding proteins. Virology  310: 64-71.

Burch, A. D.,  Fane, B. A. (2000) Foreign and chimeric external scaffolding proteins as inhibitors of Microviridae morphogenesis. J. Virol. 74: 9347-9352.