GOKUSHOVIRUSES
Gokushoviruses are a subfamily of the Microviridae. Their hosts are obligate
intracellular bacteria, such Chlamydia and Bdellovibrio. There
exists a limited understanding of the life-cycles of viruses that
infect cells that themselves live within other
cells.
Current efforts in the two laboratories are focused on elucidating the
infection
and assembly processes of these newly discovered viruses.
Figure 5: CHP2 Virions (left) and
stain penetrated empty procapsids (right).
Current Investigators: Matthew Dubrava
Alumnus Investigators: Karie Brentlinger, Susan Hafenstein.
Collaborators: Dr. Ian Clarke's group (University of Southampton
Medical School), Dr. Mavis Agbandje-Mckenna's group (University of
Florida), Dr. Robert Mckenna's group (University of Florida)
Salim, O, Stilton, R. Cutliffe, L. Fane,
B. A., Clarke, I. N. (2008) Behind the Chlamydial Cloak, the life-cycle
of a virus Chp2 revealed. Virology 377: 440-445.
Skilton R. J., Cutcliffe L. T,
Pickett M. A., Lambden P. R. *Fane B. A. Clarke I. N. (2007).
Intracellular parasitism of chlamydiae: the specific infectivity of
chlamydiaphage Chp2, in Chlamydophila abortus. J. Bact. 189: 4957-9.
Clarke, I. N., Cutcliffe, L. T., Everson,
J. S., Garner, S. A., Lambden, P. R.. Pead, P. J., Pickett,M. A.
Brentlinger, K. L., Fane, B. A. (2004) Chlamydiaphage Chp2, a skeleton
in the øX174 closet: scaffolding protein and procapsid
identification. J. Bact. 186: 7571-4.
Garner S. A., Everson, J. S., Lambden, P.
R., Fane, B. A., Clarke I. N. (2004) Isolation, Molecular
Characterisation and Genome Sequence of a Bacteriophage (Chp3) from
Chlamydophila pecorum. Virus Genes. 28:207-14.
Everson, J. S., Garner, S. A.,
Lambden, P. R. ,Fane, B. A. Clarke, I. N. (2003)The host range of
chlamydiaphages ØCPAR39 and Chp3., J. Bacteriol. 185:6490-2.
Brentlinger, K. L., Hafenstein, S. ,
Novak, C. R. Fane, B. A. (communicating author), Borgon, R.
McKenna, R., Agbandje-McKenna, M. (2002) Microviridae, a family
divided. Isolation, characterization and genome sequence
of øMH2K, a bacteriophage of the
obligate intracellular parasitic bacterium
Bdellovibrio bacteriovorus. J. Bacteriol. 184:1089-94.
Everson, J.S., Garner, S. A., Fane B. ,
Liu, B-L., Lambden, P. R., Clarke, I. N. (2002) Biological
Properties and cell tropism of Chp2, a bacteriophage of the obligate
intracellular Bacterium C. Abortus. J. Bacteriol. 184: 2748-54.
Liu, B. L., Everson, J. S., Fane, B. A.,
Giannikopoulou, P., Vretou, E., Lambden, P. R., Clarke, I. N. (2000)
The molecular characterization of a bacteriophage (Chp2) from Chlamydia
psittaci. J. Virol. 74: 3646-3469.
