David Galbraith

Professor - The School of Plant Sciences, BIO5 Institute, Biomedical Engineering, & Institute of the Environment
Keating Building, Room 341
(520) 621-9153

Educational Background

David Galbraith received his B.A., M.A. and Ph.D. degrees in Biochemistry from Cambridge University. He was a NATO Postdoctoral Fellow at Stanford University.

Research Interests

  • Dr. Galbraith's research interests include biological instrumentation, developmental and tissue-specific gene expression in eukaryotes, functional genomics and proteomics, and issues in biodiversity. He was elected a Fellow of the American Association for Advancement of Science in 2002, and served as Specialty Chief Editor of Frontiers in Genomic Assay Technology from 2011-2017. He is also an Associate Editor for Cytometry, and for Plant Methods. He was elected Secretary of the International Society for Advancement of Cytometry (I.S.A.C,) in 2016.
  • Recent funding from the National Institutes of Health(N.C.I.) was used to explore early events in gene expression during the development of ductal adenocarcinoma in the pancreas, and from N.S.F. to develop hand-held devices to track plant and human pathogens. Current funding from the Arizona Department of Health addresses early events in prostate oncogenesis, and from the United States Department of Agriculture (via the B.A.R.D. program) investigates the molecular mechanisms underlying grape bud dormancy.

Selected Publications

  • Galbraith, D.W., Harkins, K.R., Maddox, J.R., Ayres, N.M., Sharma, D.P., and Firoozabady, E. (1983). Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049-1051.
  • Afonso, C.L., Harkins, K.R., Thomas-Compton, M., Krejci, A., and Galbraith, D.W. (1985). Production of somatic hybrid plants through fluorescence activated sorting of protoplasts. Nature Biotechnology 3:811-816.
  • Harkins, K.R., and Galbraith, D.W. (1987). Factors governing the flow cytometric analysis and sorting of large biological particles. Cytometry 8:60-71.
  • Harkins, K.R., Jefferson, R.A., Kavanagh, T.A., Bevan, M.W., and Galbraith, D.W. (1990). Expression of photosynthesis related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. Proceedings of the National Academy of Sciences U.S.A. 87:816-820.
  • Bharathan, G., Lambert, G.M., and Galbraith, D.W. (1994). Nuclear DNA contents of monocotyledons and related taxa. American Journal of Botany 81:381-386.
  • Zilmer, N.A., Rodriguez, J.J., Yopp, T.A., Lambert, G.M., and Galbraith, D.W. (1995). Flow cytometric analysis using digital signal processing. Cytometry 20:102-117.
  • Galbraith, D.W., Grebenok, R.J., Lambert, G.M., and Sheen, J. (1995). Flow cytometric analysis of transgene expression in higher plants: Green Fluorescent Protein. Methods in Cell Biology 50:3-12.
  • Sheen, J., Hwang, S., Niwa, Y., Kobayashi, H., and Galbraith, D.W. (1995). Green fluorescent protein as a new vital marker in plant cells. Plant Journal 8:777-784.
  • Gong, F.-C., Giddings, T.H., Meehl, J.B., Staehelin, L.A., and Galbraith, D.W. (1996). Z-membranes: artificial organelles for over-expressing recombinant integral membrane proteins. Proceedings of the National Academy of Sciences U.S.A. 93:2219-2223.
  • Grebenok, R.J., Pierson, E.A., Lambert, G.M., Gong, F.-C., Afonso, C.L., Haldeman-Cahill, R., Carrington, J.C., and Galbraith, D.W. (1997). Green-Fluorescent Protein fusions for efficient characterization of nuclear localization signals. Plant Journal 11:573-586.
  • Grebenok, R.J., Lambert, G.M., and Galbraith, D.W. (1997). Characterization of the targeted nuclear accumulation of GFP within the cells of transgenic plants. Plant Journal 12:685-696.
  • Winkler, R.G., Frank, M.R., Galbraith, D.W., Feyereisen, R., and Feldmann, K.A. (1998). Systematic reverse genetics of Transfer-DNA-tagged lines of Arabidopsis: isolation of mutations in the cytochrome P450 gene superfamily. Plant Physiology 118:743-750.
  • Chytilova, E., Macas, J., Sliwinska, E., Rafelski, S., Lambert, G., and Galbraith, D.W. (2000). Nuclear dynamics in Arabidopsis thaliana. Molecular Biology of the Cell 11:2733-2741.
  • Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., Galbraith, D.W., and Bohnert, H.J. (2001). Gene expression profiles during the initial phase of salt stress in rice (Oryza sativa L.). Plant Cell 13:889-906.
  • Xu, W., Bak, S., Decker, A., Paquette, S.M., Feyereisen, R., and Galbraith, D.W. (2001). Microarray-based analysis of gene expression in very large gene families: the Cytochrome P450 gene superfamily of Arabidopsis thaliana. Gene 272:61-74.
  • Zhang, X., Zhang, L., Dong, F., Gao J., Galbraith, D.W., and Song, C.-P. (2001). Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiology 126:1438-1448.
  • Birnbaum, K., Shasha, D.E., Wang, J.Y., Jung, J.W., Lambert, G.M., Galbraith, D.W., and Benfey, P.N. (2003). A gene expression map of the Arabidopsis root. Science 302:1956-1960.
  • Song, C-P., Guo, Y., Qiu, Q., Lambert, G., Galbraith, D.W., Jagendorf, A, and Zhu, J.-K. (2004). A probable Na+/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A. 101:10211-10216.
  • Kristensen, C., Morant, M., Olsen, C.E., Galbraith, D.W., Lindberg-Møller, B., and Bak, S. (2005). Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome. Proc. Natl. Acad. Sci. U.S.A. 102:1779-1884.
  • Zanetti, M.E., Chang, I.-F., Gong, F.C., Galbraith, D.W., and Bailey-Serres, J. (2005). Immunopurification of polyribosomal complexes of arabidopsis for global analysis of gene expression. Plant Physiology 138:624-635.
  • Birnbaum, K., Jung, J.W., Wang, J.Y., Lambert, G.M., Hirst, J.A., Galbraith, D.W., and Benfey, P.N. (2005). Cell-type specific expression profiling in plants using fluorescent reporter lines, protoplasting, and cell sorting. Nature Methods 2:1-5.
  • LaJeunesse, T.C., Lambert, G., Andersen, R.A., Coffroth, M.A., and Galbraith, D.W. (2005). Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates. Journal of Phycology 41:880-886.
  • Zhang, C.Q, Gong, F.C., Lambert, G.M., and Galbraith, D.W. (2005). Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. Plant Methods 2005, 1:7 doi:10.1186/1746-4811-1-7.
  • Song, C-P., and Galbraith, D.W. (2006). AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis. Plant Molecular Biology 60:241-257.
  • Rangwala, S.H., Elumalai, R., Vanier, C., Ozkan, H., Galbraith, D.W., and Richards, E.J. (2006). Meiotically-stable natural epigenetic variation in Sadhu, a novel Arabidopsis retroposon. P.L.o.S. Genetics 2:270-281.
  • Li, J., Li, X., Su, H., Chen, H., and Galbraith, D.W. (2006). A framework of integrating gene relations from heterogeneous data sources: an experiment on Arabidopsis thaliana. Bioinformatics 22:2037-2043.
  • Galbraith, D.W. (2006). The daunting process of MIAME. Nature 444:31.
  • Kris, R.M., Felder, S., Deyholos, M., Lambert, G.M., Botros, I., Martel, R., Seligmann, B., and Galbraith, D.W. (2007). High-throughput, high-sensitivity analysis of gene expression in Arabidopsis thaliana. Plant Physiology 144:1256-1266.
  • Barthelson, R.A., Lambert, G.M., Vanier, C., Lynch, R.M., and Galbraith, D.W. (2007). Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells. B.M.C. Genomics 8:340.
  • Zhang, C.Q., Barthelson, R.A., Lambert, G.M., and Galbraith, D.W. (2008). Characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. Plant Physiology 147:30-40.
  • Edwards, J.D., Sweeney, M., Janda, J., Gaikwad, A., Liu, B., Leung, H., and Galbraith, D.W. (2008). Development of a high-throughput, low-cost genotyping platform based on oligonucleotide microarrays, and its evaluation in rice. Plant Methods 2008, 4:13.
  • Galbraith, D.W. (2009). Simultaneous flow cytometric quantification of plant nuclear DNA contents over the full range of described angiosperm 2C values. Cytometry 75A:692-698.
  • Mustroph, A., Zanetti, M.E., Jang, C.J.H., Galbraith, D.W., Girke, T., and Bailey-Serres, J. (2009). Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 106:18849-18854.
  • Zárate, X., Henderson, D.C., Phillips, K.C., Lake, A.D., and Galbraith, D.W. (2010). Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts. Proteome Science 2010, 8:32 doi:10.1186/1477-5956-8-32.
  • Liu, Y., Zhu, X.Y., Zhang, S., Bernardo, M., Edwards, J.D., Galbraith, D.W., Leach, J., Zhang, G., Liu, B., and Leung, H. (2011). Dissecting quantitative resistance against blast using heterogeneous inbred families in rice. Theoretical and Applied Genetics 122:341-353.
  • Galbraith, D.W., Janda, J., and Lambert, G.M. (2011). Multiparametric analysis, sorting, and transcriptional profiling of plant protoplasts and nuclei according to cell type. Methods in Molecular Biology 699:407-429.
  • Krishnan, A.R., Sweeney, M., Vasic, J., Galbraith, D.W., and Vasic, B. (2011). Barcodes for DNA sequencing with guaranteed error correction capability. Electronics Letters 47:236-237.
  • Bourzac, K.M., Rounseville, M.P., Zarate, X., Maddula, V.S.R.K., Henderson, D.C., Luckey, J., Seligmann, B., and Galbraith, D.W. (2011). A high-density quantitative nuclease protection microarray platform for high throughput analysis of gene expression. Journal of Biotechnology 154:68-75.
  • Kimzey, M.J., Zarate, X., Galbraith, D.W. and Lau, S.S. (2011). Optimizing microarray-based in situ transcription-translation of proteins for MALDI mass spectrometry. Analytical Biochemistry 414:282–286.
  • Galbraith, D.W. (2011). Frontiers in Genomic Assay Technologies: the grand challenges in enabling data-intensive biological research. Frontiers in Genetics 2:26. doi: 10.3389 / fgene.2011.00026.
  • Galbraith, D.W., Bennetzen, J.L., Kellogg, E.A., Pires, J.C., and Soltis, P.S. (2011). The genomes of all angiosperms: a call for a coordinated global census. Journal of Botany Article 646198 doi:10.1155/2011/646198.
  • Baisakh, N., Ramana Rao, M. V., Rajasekaran, K., Subudhi, P., Janda, J., Galbraith, D., Vanier, C. and Pereira, A. (2012). Enhanced salt stress tolerance of rice plants expressing a vacuolar H+-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel. Plant Biotechnology Journal 10:453–464.
  • Zhang, C.Q., and Galbraith, D.W. (2012). RNA interference-mediated gene knockdown within specific cell types. Plant Molecular Biology 80:169-176.
  • Galbraith, D.W. (2012). Flow Cytometry and Cell Sorting: the Next Generation (guest volume editor). Methods Volume 57.
  • Wang, Z., Hobson, N., Galindo, L., McDill, J., Hawkins, S., Neutelings, G., Datla, R., Lambert, G., Galbraith, D.W., Cullis, C., Wong, G.K.-S., Wang J., and Deyholos, M.K. (2012). The genome of flax (Linum usitatissimum) assembled de novo from short shotgun sequence reads. Plant Journal 14:461-473.
  • Qin, J., Scheuring, C.F., Wei, G., Zhi, H., Zhang, M., Huang, J.J., Zhou, X., Galbraith, D.W., and Zhang, H.-B. (2013). Identification and characterization of a repertoire of genes differentially expressed in developing top ear shoots between a superior hybrid and its parental inbreds in Zea mays L. Molecular Genetics and Genomics 288:691-705.
  • Grindberg, R.V., Yee-Greenbaum, J.L., McConnell, M.J., Novotny, M., O’Shaughnessy, A.L., Lambert, G.M., Araúzo-Bravo, M.J., Lee, J., Fishman, M., Lin, X., Robbins, G.E., Lin, X., Venepally, P., Badger, J.H., Galbraith, D.W., Gage, F.H., and Lasken, R.S. (2013). RNA-Seq from single nuclei. Proceedings of the National Academy of Sciences U.S.A. 110:19802-19807.
  • Galbraith, D.W. (2014). Flow cytometry and sorting in Arabidopsis. In: Arabidopsis Protocols (3rd. edition; J.J.S. Serrano, ed.). Methods in Molecular Biology 1062:509-537.
  • Galbraith, D.W. (2014). Endoreduplicative standards for calibration of flow cytometric C-value measurements. Cytometry 85A:368-374.
  • Chen, S.-C., Cannon, C.H., Kua, C.-S., Liu, J.J., and Galbraith, D.W. (2014). Genome size variation in the Fagaceae and its implications for trees. Tree Genetics and Genomes 10:977-988.
  • Mittal, A., Balasubramanian, R., Cao, J., Singh, P., Subramanian, S., Hicks, G., Nothnagel, E.A., Abidi, N., Janda, J., Galbraith, D.W., and Rock, C.D. (2014). TOPOISOMERASE 6B is involved in chromatin remodeling associated with hormone and environmental control of carbon partitioning, secondary metabolite and cell wall synthesis, and epidermal morphogenesis in Arabidopsis. Journal of Experimental Botany 65:4217-4239.
  • Galbraith, D.W. (2014). Challenges and solutions in cytometric measurements of non-mammalian species. Cytometry 85A:831-832.
  • Zarate, X., and Galbraith, D.W. (2014). A cell-free expression platform for production of protein microarrays. Methods in Molecular Biology 1118:297-307.
  • Naivar, M.A., and Galbraith, D.W. (2015). Digital data acquisition and processing. Current Protocols in Cytometry 71:10.19.1-10.19.12.
  • Galbraith, D.W. (2015). Redrawing the frontiers in the age of post-publication review. Frontiers in Genetics: doi: 10.3389/fgene.2015.00198.w
  • Cantu-Bustosa, J.E., Vargas-Corteza, T., Morones-Ramireza, J.R., Balderas-Renteria, I., Galbraith, D.W., McEvoy, M.M., and Zarate X. (2016). Expression and purification of recombinant proteins in Escherichia coli tagged with the metal-binding protein CusF. Protein Expression and Purification doi: 10.1016/j.pep.2016.01.007.
  • Samadder, P., Weng, N., Doetschman, T.C., Heimark, R., and Galbraith, D.W. (2016). Flow cytometry and nuclear sorting for Cre-based analysis of changes in transcriptional states. Cytometry 89:430-442.
  • Canady, M., Clark, C., Smith, R., Larsen, C., Gutierrez, A., Janda, J., Galbraith, D.W., Blaszczak, A.G., and Wozniak, E.M. (2016). How the novel biostimulant CYT14 influences nutrient uptake of common foliar nutritional supplements as evidenced by genomics and ICP analysis. Acta Horticulturae 1148:69-75.
  • Blaszczak, A.G., Smith, R., Gutierrez, A., Galbraith, D.W., Janda, J., Vanier, C., and Wozniak, E.M. (2016). Molecular mechanism of action for the novel biostimulant CYT31 in plants exposed to drought stress. Acta Horticulturae 1148:85-92.

Teaching Responsibilities

  • PLS539: Methods In Cell Biology and Genomics
  • PLS195: Plant Sciences First Year Colloquium
  • HNRS195: Honors First Year Colloquium

Areas of Research

  • Biochemistry and Physiology
  • Biodiversity and Evolutionary Biology
  • Cell and Developmental Biology
  • Environmental and Stress Biology
  • Genetics and Epigenetics
  • Genomics, Bioinformatics, and Systems Biology

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